Will Lung Tissue Repair Itself Air Leak

. 2014 January;12 Suppl 1(Suppl 1):s235-42.

doi: x.2450/2013.0214-12. Epub 2022 May 14.

Pleural tissue repair with string blood platelet gel

Affiliations

• PMID: 23736928
• PMCID: PMC3934292
• DOI: 10.2450/2013.0214-12

Gratuitous PMC commodity

Pleural tissue repair with cord blood platelet gel

Lorenzo Rosso  et al. Blood Transfus. 2014 Jan .

Costless PMC article

Abstruse

Groundwork: Prolonged air leak is the major crusade of morbidity afterward pulmonary resection. In this report nosotros used in vitro and in vivo experiments to investigate an innovative approach based on the use of homo umbilical cord blood platelet gel.

Materials and methods: In vitro, a scratch assay was performed to examination the tissue repair capability mediated by cord blood platelet gel compared to the standard culture conditions using man primary mesothelial cells. In vivo, an iatrogenic injury was made to the left lung of 54 Wistar rats. Cord blood platelet gel was placed on the injured area only in treated animals and at different times histological changes and the presence of pleural adhesions were evaluated. In addition, changes in the pattern of soluble inflammatory factors were investigated using a multiplex proteome array.

Results: In vitro, mesothelial cell damage was repaired in a shorter time by cord blood platelet gel than in the control condition (24 versus 35 hours, respectively). In vivo, formation of new mesothelial tissue and consummate tissue recovery were observed at 45±ane and 75±1 hours in treated animals and at 130±2.five and 160±half-dozen hours in controls, respectively. Pleural adhesions were axiomatic in 43% of treated animals compared to 17% of controls. No complications were observed. Interestingly, some crucial soluble factors involved in inflammation were significantly reduced in treated animals.

Discussion: Cord claret platelet gel accelerates the repair of pleural harm and stimulates the development of pleural adhesions. Both properties could be particularly useful in the management of prolonged air leak, and to reduce inflammation.

Figures

Figure 1

The effects of FBS and CB platelet releasate on mesothelial repair in a scratch analysis. (A) Reconstitution of mesothelial lining in the presence of FBS (standard status, northward =5) at T0, T8, T16, T24, T35; (B) Reconstitution of mesothelial lining in the presence of CB platelet releasate (n =v) at T0, T8, T16, T24; (C) Information are represented as mean ± SD. The P value was obtained performing an unpaired t-exam. *P <0.0001.

Figure 2

TNF-α, IFN-γ and IL-two concentrations in rat sera at 75 hours after pleural damage (control group, CG) and after CBPG handling (treatment grouping, TG). Inflammatory factors were measured by proteome assay (pg/mL; CG n =four, TG due north =six; median and range). The P value was obtained performing an unpaired t-test. *P <0.0001; **P <0.0003.

Figure 3

Presence of a pleural adhesion on the thoracotomy.

Figure 4

Histological sections of rat lung in the two different groups. On the left side the treated animals: (A) recent subpleural damage characterised by marked haemorrhagic extravasation, fibrin-rich clot bleeding associated with acute and chronic inflammation and fibroblast hyperplasia; (B) subpleural damage with a visible new mesothelial lining; (C) subpleural damage no longer visible. Complete mesothelial repair; (D) late subpleural damage in presence of weakly basophilic material due to Alcian blue 8GX staining. On the right side the controls: (East) recent subpleural harm characterised by marked haemorrhagic extravasation, fibrin-rich clot bleeding associated with acute inflammation; (F) subpleural damage with leucocyte-fibrin clot associated with scattered scar-like components; (G) subpleural damage in an advanced stage of mesothelial tissue repair; (H) subpleural harm no longer visible. Complete mesothelial repair.

Figure five

Representative trends of tissue damage and repair in treated and control animals over time (hours): (A) surgical injury, (B) strongly damaged, (C) withal evident damage, (D, bold grey line) initial lining, (E) axiomatic lining, (F, assuming grey line) complete tissue repair.

Like articles

• First endocavitary handling with cord blood platelet gel for perianal fistula.

  Bisceglia G, Santodirocco 1000, Faienza A, Mastrodonato N, Urbano F, Totaro A, Bazzocchi F, Mauro LD. Bisceglia G, et al. Regen Med. 2022 Jan;15(ane):1171-1176. doi: 10.2217/rme-2019-0074. Epub 2022 Feb 12. Regen Med. 2022. PMID: 32046600

• Extensive Characterization of Platelet Gel Releasate From Cord Blood in Regenerative Medicine.

  Parazzi 5, Lavazza C, Boldrin V, Montelatici E, Pallotti F, Marconi M, Lazzari L. Parazzi Five, et al. Cell Transplant. 2022;24(12):2573-84. doi: 10.3727/096368915X687471. Epub 2022 Feb eighteen. Cell Transplant. 2022. PMID: 25695232

• Effect of Cord Blood Platelet Gel on wound healing chapters of homo Mesenchymal Stromal Cells.

  Mallis P, Alevrogianni V, Sarri P, Velentzas AD, Stavropoulos-Giokas C, Michalopoulos E. Mallis P, et al. Transfus Apher Sci. 2022 Jun;59(3):102734. doi: 10.1016/j.transci.2020.102734. Epub 2022 Jan 27. Transfus Apher Sci. 2022. PMID: 32005441

• Cord blood platelet gel for the treatment of inherited epidermolysis bullosa.

  Gelmetti A, Greppi Due north, Guez Due south, Grassi F, Rebulla P, Tadini G. Gelmetti A, et al. Transfus Apher Sci. 2022 Jun;57(3):370-373. doi: ten.1016/j.transci.2018.05.021. Epub 2022 May 10. Transfus Apher Sci. 2022. PMID: 29933907 Review.

• Use of platelet growth factors in treating wounds and soft-tissue injuries.

  Rozman P, Bolta Z. Rozman P, et al. Acta Dermatovenerol Alp Pannonica Adriat. 2007 December;xvi(4):156-65. Acta Dermatovenerol Alp Pannonica Adriat. 2007. PMID: 18204746 Review.

Cited by 2 articles

• String Blood Platelet Gel as a Treatment of Occipital Pressure level Injuries in Newborns: Report of Ii Cases.

  Ferrario S, Zorz A, Sorrentino Thou, Villa S, Cavalli R, Mosca F, Plevani L, Ghirardello Due south. Ferrario South, et al. Children (Basel). 2022 Nov 23;viii(12):1079. doi: 10.3390/children8121079. Children (Basel). 2022. PMID: 34943275 Costless PMC article.

• Human being cord claret-derived platelet lysate enhances the therapeutic activity of adipose-derived mesenchymal stromal cells isolated from Crohn's disease patients in a mouse model of colitis.

  Forte D, Ciciarello M, Valerii MC, De Fazio L, Cavazza E, Giordano R, Parazzi V, Lazzari L, Laureti Southward, Rizzello F, Cavo M, Curti A, Lemoli RM, Spisni E, Catani L. Forte D, et al. Stem Jail cell Res Ther. 2022 Sep 9;6(1):170. doi: 10.1186/s13287-015-0166-2. Stem Cell Res Ther. 2022. PMID: 26353774 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Archivio Istituzionale della Ricerca Unimi
  • Data Conversion Labs
  • Europe PubMed Central
  • PubMed Central

• Medical

  • ClinicalTrials.gov

Source: https://pubmed.ncbi.nlm.nih.gov/23736928/

Posted by: ketchumdincestamed58.blogspot.com

Share this post